THE EFFECT OF BALI CATTLE SPERMATOZOA USING VARIOUS SOURCES OF CARBOHYDRATES WITH EGG YOLK SKIMMED EXTENDER

Armayani Armayani, Angga Nugraha, Muh. Irwan, Reski Amalia Burhanuddin, Sinta Safitri, Wahid Wahid

Abstract


A study has been conducted on the effect of Bali cattle spermatozoa using various sources of carbohydrates with egg yolk skimmed extender. The method used was Artificial Insemination to collect semen, with 4 semen extender preparation processes involving the addition of egg yolk skim, namely: the preparation of the skim buffer, the preparation of antibiotic buffer A consisting of 90% skim buffer with antibiotic mixed with egg yolk at 10% of the total extender A, and the preparation of extender B consisting of 72% skim antibiotic buffer, 10% egg yolk, 16% glycerol, and each treatment was added with 2% glucose and 2% fructose. The data obtained showed no significant differences (p>0.05) between the administration of 2% glucose, 2% fructose, and 2% sucrose added to each egg yolk skim extender, in terms of sperm motility and the percentage of live spermatozoa.

Keywords: Artificial Insemination, Carbohydrates, Bali Cattle, Egg Yolk Skim, Spermatozoa


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DOI: https://doi.org/10.35334/jpen.v7i2.5957

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